真菌菌丝体培养和提取DNA方法的改进

真菌菌丝体培养和提取DNA方法的改进

AN IMPROVED PROTOCOL FOR FUNGAL DNA PREPARATION

何月秋 

  DNA from fungal mycelia can be inexpensively prepared in a microfuge tube scale.The small scale of the procedure described here makes it possible for one people to conveniently handle up to 60 samples in less than 5 hours and obtain l0~50g of DNA per sample,which is equal to the amount enought for several Southem hybridizations and 500~2500 PCR-based reactions.

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作者单位:何月秋(云南农业大学植物病理重点实验室,昆明 650201) 

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