灰树花总DNA的制备及基因组文库的构建

论文题目: 灰树花总DNA的制备及基因组文库的构建A Method for Preparation of Genomic DNA From Grifola frondosa and Construction of a Genomic Library

  作者姓名: 徐志祥, 程 度, 李宝健Xu Zhi-Xiang, Cheng Du, Li Bao-Jian

  工作单位: 基因工程国家教育部重点实验室∥中山大学生命科学院,广州 510275Key Laboratory of Genetic Engineering of Ministry of Education ∥ School of Life Sciences , Sun Yat-sen University Guangzhou 510275, China

  摘  要: 灰树花是一种珍贵的药用真菌,因为多糖含量较高,较难获得高质量的总DNA,本文提出了一种制备高质量灰树花总DNA及构建灰树花基因组文库的方法。该方法制备的灰树花总DNA,经Sau3AI酶切后,用于构建基因组文库,可得到2×105个转化子/50mg,平均插入片段为14kb。本研究为下一步克隆灰树花中的基因以及进行其他分子生物学研究奠定了基础。Abstract: Grifola frondosa, is a valuable medicinal fungus. High quality total genomic DNA is difficult to prepare due to its high polysaccharide content. A method for the preparation of Grifola frondosa total genomic DNA and construction of Grifola frondosa, genomic library is described. Genomic DNA prepared by this method is digested by Sau3A I restriction enzyme. Constructed genomic library give a titer of 2×105 transformants/50mg , with a average insert size of 14kb. This has paved way for the cloning of other Grifola frondosa genes and molecular biology studies.

  关 键 词: 灰树花 ;DNA提取 ; 基因组文库 Key words:Grifola frondosa ; DNA preparation ; Genomic library

  发表年份:《遗传》2004

  卷(期): 26(5)

  起止页数: 711-713

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